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Image Search Results
Journal:
Article Title: An ADP-Ribosylation Factor GTPase-activating Protein Git2-short/KIAA0148 Is Involved in Subcellular Localization of Paxillin and Actin Cytoskeletal Organization
doi:
Figure Lengend Snippet: Subcellular fractionation of endogenous Git2-short. 3Y1 cells were subjected to subcellular fractionation as described in MATERIALS AND METHODS. Total, soluble, and particulate fractions (20 μg each) were separated by SDS-PAGE and immunobloted with anti-Git2-short, anti-paxillin, anti-fibroblast growth factor R1 (FGFR1), or anti-CrkII antibodies. Similar distributions of endogenous Git2-short in NIH3T3 and HA-Git2-short in 3Y1 or in NIH3T3 were also observed (Mazaki and Sabe, unpublished results).
Article Snippet: Other antibodies were purchased from commercial sources: anti-paxillin (mouse monoclonal antibody clone 349; Transduction Laboratories, Lexington, KY), anti-influenza hemagglutinin (HA; mouse monoclonal antibody clone 16B12 and polyclonal antibodies; Berkeley Antibody, Richmond, CA), anti-myc (mouse monoclonal antibody clone 9E10, and polyclonal antibodies; Berkeley Antibody), anti-green fluorescent protein (GFP) (mouse monoclonal antibody clone B34, Berkeley Antibody), anti-GST (mouse monoclonal antibody clone 4C10, Berkeley Antibody),
Techniques: Fractionation, SDS Page